Thermo Scientific DH5α Competent Cells for Subcloning are chemically competent E. coli cells recommended for routine subcloning into plasmid vectors middle. Subcloning Efficiency Cells are not suitable for generating cDNA libraries. The φ80dlacZΔM15 marker provides an α-complementary β-galactosidase gene from a pUC or similar vector and can be used for blue-white colony selection on bacterial agar plates containing Bluo-Gal or X-Gal.

• Transformation efficiency: >1x10⁶ cfu/µg pUC19 DNA
• Suitable for routine cloning applications
• Convenient economical packaging
• Contains Genetic markers for blue-white colony screening

Applications
DH5α competent cells for subcloning are suitable for standard DNA cloning applications:
•PCR or restriction enzyme digestion reactions Post-DNA cloning
• Subcloning (transfer DNA from parental vector to destination vector)
• Plasmid isolation

Genotype
F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ- thi–1 gyrA96 relA1