Thermo Scientific DH5α Competent Cells are high-efficiency, chemically competent E. coli cells suitable for constructing gene libraries using plasmid-derived vectors or Generation of cDNA libraries. The φ80dlacZΔM15 marker provides an α-complementary β-galactosidase gene from a pUC or similar vector and can be used for blue-white colony selection on bacterial agar plates containing Bluo-Gal or X-Gal. RecA1 and endA1 mutations in DH5α cells improve insert stability and quality of extracted plasmid DNA.

•High transformation efficiency: >1x10⁹ cfu/µg pUC19 DNA
• Suitable for routine cloning and high-throughput cloning applications
• Ability to perform blue-white colony formation Gene markers for screening
• Easy to use, two reactions per bottle
• Contains SOC external growth medium

Applications
DH5α competent cells, suitable for a variety of applications requiring high transformation efficiency:
• Efficient DNA cloning from PCR, cDNA generation reactions
• recA1 marker facilitates easy transformation of DNA
• Suitable for use with plasmid-derived vectors Generation of cDNA libraries
• Site-directed mutagenesis

Genotype
F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ- thi–1 gyrA96 relA1