Thermo Scientific DH10B Competent Cells are high-efficiency, chemically competent E. coli cells. This DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine and methyladenine, and can efficiently clone prokaryotic and eukaryotic genomic DNA. These cells are suitable for constructing gene libraries or generating cDNA libraries using plasmid-derived vectors. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors and can be used for blue-white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal.

•High transformation efficiency: >1x10⁹ cfu/µg pUC19 DNA
• Suitable for routine and high-throughput cloning applications
• Blue-white colony screening is possible
•Easy to use, each bottle can be packaged for two reactions
•Contains SOC external growth medium

Applications
DH10B competent cells are suitable for applications requiring high Multiple applications for transformation efficiency:
• Efficient DNA cloning from prokaryotic and eukaryotic sources
• Large plasmid and BAC cloning
• Suitable for cDNA library generation using plasmid-derived vectors
• Site-directed mutagenesis< br>
Genotype
F–mcrAΔ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ (ara-leu)7697 galU galKλ–rpsL(Str< sup>R) nupG

Note: Optimized for use with CloneJET PCR Cloning Kit (Cat. No. K1231, K1232) and Phusion Site-Directed Mutagenesis Kit (Cat. No. F541) , and combined versions (item numbers K123120, K123240, F542) are available.