Thermo Scientific M.SssI can target unmethylated or hemimethylated in a 5'-CpG-3' background All cytosine nucleotide bases in double-stranded DNA are methylated at the C5 position.
This enzyme is specially formulated for fast reaction times without affecting reaction efficiency. The enzyme completes the modification of all CpGs in 15 minutes at 37°C. In addition, the enzyme was specifically validated for genomic DNA, a major substrate in epigenetic research.

M.SssI provides optimized 10X reaction buffer and 50X S-adenosylmethionine (SAM) as a cofactor.

Product Advantages

•Efficient – Complete in vitro methylation of all CpGs in unmethylated and hemimethylated DNA
•Fast – Reaction completes in 15 minutes at 37°C
•Stable – can be stored for 2 years at -20°C

Applications

•Epigenetic research
•DNA methylation in vitro for methylation analysis
•Identification of overlapping CpG sequences inhibiting endonucleases.
•[3H] Labeled DNA

This enzyme labels all cytosine nucleotide bases in unmethylated or hemimethylated double-stranded DNA in a 5'-CpG-3' background The C5 position of the base moiety is methylated.

Thermo Scientific M.SssI is formulated for fast reaction times without compromising reaction efficiency. The enzyme completes the modification of all CpGs in 15 minutes at 37°C. In addition, the enzyme was specifically validated for genomic DNA, a major substrate in epigenetic research.

M.SssI provides optimized 10X reaction buffer and 50X S-adenosylmethionine (SAM) as a cofactor.

Product Advantages

Efficient – complete in vitro methylation of all CpGs in unmethylated and hemimethylated DNA
Fast – reaction in Completed in 15 minutes at 37°C
Stable – 2 years at -20°C

Applications

•Epigenetic research
•DNA in vitro Methylation for Methylation Analysis
•Identifies overlapping CpG sequences that inhibit endonucleases.
• [3H] Labeled DNA