Thermo Scientific Proteinase K, available in 5 x 1 mL aliquots, is an enzyme capable of opening aliphatic, aromatic or hydrophobic An enzyme that breaks down the peptide bond at the carboxyl terminus of amino acids. Proteinase K belongs to the serine protease family. This product can hydrolyze the smallest tetrapeptide.

Product Features

• Ready-to-use solution
• Compatible with a wide range of reaction conditions

Applications< br>
• Isolating genomic DNA from mouse tail
• Isolating genomic DNA from cultured cells
• Removing DNase and RNase during isolation of DNA and RNA from tissues or cell lines
• Determine Enzyme Location
• Improve cloning efficiency of PCR products

Notes

• The recommended working concentration of proteinase K is 0.05 to 1mg/mL. 0.2 to 1% SDS or 1 to 4M urea can increase enzyme activity
• Ca²⁺ can protect proteinase K against autolysis, increase thermal stability and regulate proteinase K substrates Binding site functionality
• Stable over a wide pH range: pH 4.0 to 12.5, with optimal activity at pH 7.5 to 8.0
• • Optimal at 50 to 55°C Activity
• • Rapidly denatures at temperatures above 65°C