Thermo Scientific WELQut Protease is a highly specific recombinant serine protease from Staphylococcus aureus. It recognizes and precisely cleaves recombinant proteins containing engineered recognition sequences † W- E- L- Q↓X (Trp, Glu, Leu, Gln, X can be any amino acid). The protease cleaves outside the recognition sequence, leaving no additional amino acids bound to the target protein.

WELQut protease is active over a wide range of temperatures (4 to 30°C) and pH (pH 6.5 to 9.0) and does not require specific buffers.

In addition, this new protease offers several procedural advantages - it is suitable for on-column proteolysis reactions and can be easily removed from the reaction mixture using a built-in His-tag.

Product Advantages

•Cleaving outside the WELQ recognition sequence will not cause additional amino acids to bind to the target protein
•For homologous recognition sites Highly specific and does not generate non-specific product bands even after long incubations and excess protease use
• Can be easily removed from the reaction mixture using the built-in His- tag
• Suitable for columns Proteolytic Reaction

Applications

•Remove N-terminal fusion tags from recombinant protein preparations.

Note

†This cleavage sequence is present in the expression vector pLATE52, included in Thermo Scientific aLICator LIC Cloning and Expression Kit 4 (N-Terminal His Tag/WELQut) available from Thermo Scientific (#K1281).