PCR Master Mix contains Taq DNA polymerase, dNTPs and all the DNA template required for PCR and a 2X concentrated solution of all components except primers. This premixed preparation saves time and reduces contamination by reducing the pipetting steps required for PCR setup. This reagent mix is optimized for efficient and reproducible PCR.

Product Features

• Thermal Stability—half-life over 40 minutes at 95°C
• Amplification Obtain PCR product with 3'-dA end
• Provide two buffers—10X Taq 10x Taq containing KCL i> buffer and 10x Taq buffer containing (NH₄)₂SO₄. The latter can perform PCR under a wide range of magnesium ion concentrations and reduce non-specific binding
• Can bind modified nucleotides (for example: biotin-labeled nucleotides, digoxigenin Labeled nucleotides, fluorescently labeled nucleotides)

Applications

• Conventional PCR amplification, can amplify DNA fragments up to 5 kb< br>• High-throughput PCR
• DNA tag

Contains

Taq DNA polymerase (recombinase ):

• Taq DNA polymerase 5 U/μL
• 10X Taq buffer containing KCl
• 10X Taq buffer containing (NH₄)₂SO₄
• 25 mM MgCl₂

Taq DNA polymerase (recombinase) LC:

• Taq DNA polymerase 1 U/μL
• 10X Taq buffer containing KCl
• Contains (NH ₄)₂SO₄ in 10X Taq buffer
• 25 mM MgCl₂

PCR master mix (2X):

• Taq DNA polymerase (0.05 U/μL), reaction buffer, 4 mM MgCl₂ and 0.4 mM each of dNTP
• Nuclease-free water

Notes

• In PCR reactions , the error rate of Taq polymerase is 2.2 x 10^-5 mismatches for a single nucleotide per cycle. The corresponding PCR reaction accuracy is 4.5 x 10⁴. Accuracy is the reciprocal of the mismatch rate, a parameter that reflects the average number of correctly incorporated nucleotides before a single mismatch event occurs.
• Detergent-free 10X Taq buffer is recommended for microarray experiments.