Thermo Scientific Maxima Reverse Transcriptase (RT) is produced through in vitro evolution of M-MuLV RT . This enzyme not only has RNA-dependent and DNA-dependent polymerase activities, but also has RNase H activity. The engineered enzyme has significantly improved thermal stability and robustness and increased synthesis rate compared to wild-type M-MuLV RT.

Maxima Reverse Transcriptase enables reproducible cDNA synthesis at higher temperatures (50 to 65°C) from a wide range of total RNA loading amounts (1 pg to 5 µg). This makes this enzyme an ideal tool for two-step RT-qPCR (see Supporting Data).

Due to its high thermal stability, Maxima enzyme maintains full activity during the entire reverse transcription reaction, produces a higher yield of cDNA, and can even synthesize RNA transcripts up to 20 kb. Reaction temperatures can be increased to 65°C to efficiently transcribe RNA regions with complex secondary structure, or use gene-specific primers to increase specificity. Due to its increased rate of synthesis, the reverse transcriptase reaction can be completed in 15 to 30 minutes.

Product Advantages

• Obtain high yield of full-length cDNA up to 20 kb
• The upper limit of activity temperature reaches 65°C
• Thermal stability—90% activity after 60 minutes of incubation at 50°C
• High sensitivity—enables reproducible cDNA synthesis from a wide range of total RNA amounts (1 pg to 5 µg)
•Efficient—cDNA synthesis in 15 to 30 minutes
•Integration of modified nucleotides

Applications

•Two-step RT-PCR
• Two-step RT-qPCR
• First-strand cDNA synthesis
• Construction of full-length cDNA libraries
• DNA labeling
• Primer extension

Also available: for RT- Maxima first-strand cDNA synthesis kit for qPCR