5'

C

T

G

A

A

G

N16

↓

3'

3'

G

A

C

T

T

C

N14

↑

5'

Thermo Scientific Eco57I (AcuI) restriction endonuclease recognizes CTGAAG( 16/14)^ site, cleaves better in (+SAM) buffer at 37°C. See Reaction Conditions for Restriction Enzymes table to learn about the enzymatic activity of this enzyme and other restriction enzymes , double enzyme digestion conditions and heat inactivation. Note: FastDigest enzyme for rapid DNA digestion is also available. Isoschizase: AcuI.

Thermo Scientific Conventional Restriction Endonucleases are a large collection of high quality restriction enzymes optimized to function in one buffer of a five-buffer system. In addition, a universal Tango buffer is provided to facilitate double enzyme digestion. All enzymes demonstrated 100% activity under recommended buffers and reaction conditions. To ensure consistent performance, Thermo Scientific Restriction Enzyme Reaction Buffers contain premixed BSA, which enhances the stability of multiple enzymes and binds contaminants introduced into the DNA preparation.

Features

• Superior quality—strict quality control and industry-leading production processes
• Convenient color-coded five-buffer system
• Includes universal Tango buffer for double digestion
• Premix BSA in reaction buffer
• Choose from a variety of restriction endonuclease specificities

< b>Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blot
• Restriction fragment length polymorphism ( RFLP)
• SNP

Note:Eco57I requires only Mg2+ to be active but is stimulated by S-adenosylmethionine. 0.01 mM S-adenosylmethionine increases Eco57I activity 100-fold. Nonetheless, complete cleavage of some substrates with Eco57I remains elusive. The Eco57I concentration is determined by the maximum level of cleavage achievable (i.e. further increasing the amount of enzyme does not cause any change in the cleavage profile). Star activity may occur at low salts, high concentrations of glycerol (> 5%), pH values >8.0, or excess enzyme. Eco57I may still be associated with cleaved DNA. This can cause DNA band drift during electrophoresis. To avoid atypical DNA banding patterns, use 6X DNA loading dye with SDS solution for sample preparation, or heat digested DNA in the presence of SDS before electrophoresis. See product specifications for information on methylation sensitivity.