5'

C

T

G

G

A

G

N16

↓

3'

3'

G

A

C

C

T

C

N14

↑

5'

Thermo Scientific GsuI (BpmI) restriction enzyme recognizes CTGGAG( 16/14)^ site, cleaves best in B buffer at 30°C. For a table of enzymatic activities, double digestion conditions, and heat inactivation of this and other restriction enzymes, see Reaction conditions for restriction enzymes. Note: FastDigest enzyme for rapid DNA digestion is also available. Isoschizase: BpmI.

Thermo Scientific Conventional Restriction Endonucleases are a large collection of high quality restriction enzymes optimized to function in one buffer of a five-buffer system. In addition, a universal Tango buffer is provided to facilitate double enzyme digestion. All enzymes demonstrated 100% activity under recommended buffers and reaction conditions. To ensure consistent performance, Thermo Scientific Restriction Enzyme Reaction Buffers contain premixed BSA, which enhances the stability of multiple enzymes and binds contaminants that may be present in DNA preparations.

Features:

• Superior quality—strict quality control and industry-leading manufacturing processes
• Convenient color-coded five-buffer system
• Includes for dual Universal Tango buffer for enzyme digestion
•Premix BSA in the reaction buffer
•A variety of restriction endonuclease specificities are available

Note:
70% active when incubated at 37°C.
GsuI requires only Mg2+ to be active but is stimulated by S-adenosylmethionine. 0.01 mM S-adenosylmethionine increased activity 2-fold.
GsuI may be ligated to the cleaved DNA. This can cause DNA band drift during electrophoresis. To avoid atypical DNA banding patterns, use 6X DNA loading dye with SDS solution for sample preparation, or heat digested DNA in the presence of SDS before electrophoresis.
GsuI is blocked by overlapping dcm methylation. To avoid dcm methylation, use dam-, dcm- strains, such as GM2163.
See product specifications for information on methylation sensitivity.