< td>

5'		G ↓	C	T	A	G	C		3'
3'			C	G	A	T	C ↑	G		5'

Thermo Scientific NheI restriction endonuclease can recognize the G^CTAGC site and has better cutting effect in Tango buffer at 37°C (isoschizoase: AsuNHI, BmtI). See Reaction Conditions for Restriction Enzymes table to learn about the enzymatic activity of this enzyme and other restriction enzymes , double enzyme digestion conditions and heat inactivation. Note: Also available as FastDigest enzyme for rapid DNA digestion.

Thermo Scientific Conventional Restriction Endonucleases are a large collection of high quality restriction enzymes optimized to function in one buffer of a five-buffer system. In addition, a universal Tango buffer is provided to facilitate double enzyme digestion. All enzymes demonstrated 100% activity under recommended buffers and reaction conditions. To ensure consistent performance, Thermo Scientific Restriction Enzyme Reaction Buffers contain premixed BSA, which enhances the stability of multiple enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—strict quality control and industry-leading production processes
• Convenient color-coded five-buffer system
•Includes universal Tango buffer for double digestion
•Pre-mixed BSA in reaction buffer
•Multiple restriction endonuclease specificities available

< b>Applications

•Molecular cloning
•Restriction site mapping
•Genotyping
•Southern blot
•Restriction fragment length polymorphism ( RFLP)
•SNP

Note: In the case of low salt, high concentration of glycerol (>5%), pH value of > 8.0 or too much enzyme, Star activity may occur. NheI is inhibited at salt concentrations above 100 mM. For complete digestion, supercoiled plasmids may require 10 times the amount of NheI required for linear DNA (for example, 10 units are required to cleave 1 µg of pBR322 DNA.) For methylation sensitivity, see product specifications.