< td>3'

5'

↓

N8

G

A

G

N5

C

T

C

C td>

N13

↓

3'

↑

N13

C

T

C

N5

G

A

A

N td>

G

N8

↑

5'

Thermo Scientific BplI restriction enzyme recognizes the ^(8/13)GAG(N)5CTC(13/8)^ site at 37°C Cutting results are best in Tango(+SAM) buffer. For a table of enzymatic activities, double digestion conditions, and heat inactivation of this and other restriction enzymes, see Reaction conditions for restriction enzymes. Note: FastDigest enzyme is also available for rapid DNA digestion.

Thermo Scientific Conventional Restriction Endonucleases are a large collection of high quality restriction enzymes optimized to function in one buffer of a five-buffer system. In addition, a universal Tango buffer is provided to facilitate double enzyme digestion. All enzymes demonstrated 100% activity under recommended buffers and reaction conditions. To ensure consistent performance, Thermo Scientific Restriction Enzyme Reaction Buffers contain premixed BSA, which enhances the stability of multiple enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—strict quality control and industry-leading production processes
• Convenient color-coded five-buffer system
•Includes universal Tango buffer for double digestion
•Pre-mixed BSA in reaction buffer
•Multiple restriction endonuclease specificities available

< b>Applications

•Molecular cloning
•Restriction site mapping
•Genotyping
•Southern blot
•Restriction fragment length polymorphism ( RFLP)
•SNP

Remarks:BplI requires only Mg2+ to be active but is stimulated by S-adenosylmethionine. 0.05 mM S-adenosylmethionine increases BplI activity more than 100-fold. Nonetheless, complete cleavage of some substrates using BplI remains elusive. The BplI concentration is determined by the maximum level of cleavage achievable (i.e., further increasing the amount of enzyme does not cause any change in the cleavage profile). See product specifications for methylation sensitivity.