< td>

5'

G

A

T

N

N ↓

N

N

A

T

C

3'< /td>

3'

C

T

A

N

N ↑

N

N < /td>

T

A

G

5'

Thermo Scientific BseJI (BsaBI) restriction enzyme recognizes the GATNN^NNATC site and has the best cutting effect in O buffer at 65°C ( Iso-cleavage enzymes: BsaBI, Bse8I). For a table of enzymatic activities, double digestion conditions, and heat inactivation of this and other restriction enzymes, see Reaction conditions for restriction enzymes. Note: FastDigest enzyme is also available for fast DNA digestion.

Thermo Scientific Conventional Restriction Endonucleases are a large collection of high quality restriction enzymes optimized to function in one buffer of a five-buffer system. In addition, a universal Tango buffer is provided to facilitate double enzyme digestion. All enzymes demonstrated 100% activity under recommended buffers and reaction conditions. To ensure consistent performance, Thermo Scientific Restriction Enzyme Reaction Buffers contain premixed BSA, which enhances the stability of multiple enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—strict quality control and industry-leading production processes
• Convenient color-coded five-buffer system
•Includes universal Tango buffer for double digestion
•Pre-mixed BSA in reaction buffer
•Multiple restriction endonuclease specificities available

< b>Applications

•Molecular cloning
•Restriction site mapping
•Genotyping
•Southern blot
•Restriction fragment length polymorphism ( RFLP)
•SNP

Note: Less than 10% active when incubated at 37°C. Over-digestion of BseJI by more than 20-fold may result in asterisk activity. Overlapping dam methylation results in BseJI cleavage being blocked. To avoid dam methylation, use dam-, dcm- strains such as GM2163. See product specifications for methylation sensitivity.