< td>

5'			↓	C	C	W	G	G		3'
3'			G	G	W	C	C ↑	5'

Thermo Scientific EcoRII restriction endonuclease recognizes the ^CCWGG site and reacts at 37°C Cutting results best in O buffer. For a table of enzymatic activities, double digestion conditions, and heat inactivation of this and other restriction enzymes, see Restrictions Reaction conditions for sexual endonuclease. Iso-cleavage enzymes: AjnI, BseBI, Bst2UI, BstNI, BstOI, Psp6I, PspGI.

Thermo Scientific Conventional Restriction Endonucleases are a large collection of high quality restriction enzymes optimized to function in one buffer of a five-buffer system. In addition, a universal Tango buffer is provided to facilitate double enzyme digestion. All enzymes demonstrated 100% activity under recommended buffers and reaction conditions. To ensure consistent performance, Thermo Scientific Restriction Enzyme Reaction Buffers contain premixed BSA, which enhances the stability of multiple enzymes and binds contaminants introduced into the DNA preparation.

Features

• Superior quality—strict quality control and industry-leading production processes
• Convenient color-coded five-buffer system
• Includes universal Tango buffer for double digestion
• Premix BSA in reaction buffer
• Choose from a variety of restriction endonuclease specificities

< b>Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blot
• Restriction fragment length polymorphism ( RFLP)
• SNP

Note: For efficient cleavage, at least two EcoRII recognition sites need to be copied. To cleave a replicated DNA substrate that only recognizes the MvaI site, the use of EcoRII isocleavage enzyme is recommended. EcoRII may bind to severed DNA. This can cause DNA band drift during electrophoresis. To avoid atypical DNA electrophoresis band patterns, please use 6X DNA loading dye and SDS solution to prepare samples, or add SDS to the DNA digestion product and heat it before electrophoresis. Enzyme activity was measured using pBR322 DNA (dcm-). Overlapping dcm methylation results in blocked EcoRII cleavage. To avoid dcm methylation, use dam-, dcm- strains, such as GM2163. See product specifications for information on methylation sensitivity.