< td> < td>

5'		G	A	A	T	G	C		3'
3'		C	T	T	A	C ↑	G		5'

Thermo Scientific Nb.Mva1269I Nickase recognizes the GAATGC site and cleaves optimally in O buffer at 37°C. For a table of enzymatic activities, double digestion conditions, and heat inactivation of this and other restriction enzymes, see Reaction conditions for restriction enzymes.

Thermo Scientific Conventional Restriction Endonucleases are a large collection of high quality restriction enzymes optimized to function in one buffer of a five-buffer system. In addition, a universal Tango buffer is provided to facilitate double enzyme digestion. All enzymes demonstrated 100% activity under recommended buffers and reaction conditions. To ensure stable endonuclease activity, BSA is added to Thermo Scientific restriction endonuclease reaction buffer, which not only improves the stability of many types of endonucleases, but also eliminates contaminants that may be present in DNA samples. Combine.

Features

• Superior quality—strict quality control and industry-leading manufacturing processes
• Convenient color-coded five-buffer system
• Includes universal Tango buffer for dual degradation
• BSA premixed in reaction buffer
• Broad range of restriction endonuclease specificities

Applications
Generate single-stranded circular DNA from in vitro supercoiled double-stranded plasmids, which can then be used to create nested deletions for DNA sequencing, site-specific mutations, etc. Vector Preparation by Ligation-Independent Cloning Method Preparation of covalently closed double-stranded linear DNA molecules.