Thermo Scientific Phusion High-Fidelity DNA Polymerase sets the "gold standard" for efficient PCR. Phusion High-Fidelity DNA Polymerase has an error rate that is 1/50 that of Taq and 1/6 that of Pfu, making it ideal for cloning and other applications requiring high fidelity. Best choice. Compared to other DNA polymerases, Phusion DNA Polymerase performs robustly, requires shorter protocols (even in the presence of PCR inhibitors), and achieves higher yields with less enzyme.

Product Advantages
•High fidelity (52XTaq)
•Short extension time enables fast PCR (15-30 s /kb)
• Robust performance with less optimization required
• Achieve high yields of PCR products with less enzyme volume
• Available in Green buffer format for converting PCR products Load directly onto gel (F-534S or F-534L)

Applications
• High-fidelity PCR
• Cloning
• Generate sequencing templates
•Amplification of complex (high GC content) templates
•Long fragment PCR (up to 20 kb)
•Mutagenesis
•High-throughput PCR
• Microarrays

< strong>Using Phusion DNA Polymerase
The annealing rules of Phusion DNA polymerase are different from those of many common DNA polymerases (such as Taq DNA polymerase). For best results, use www.thermofisher.cn/tmcalculator.