Thermo Scientific Rapid DNA Ligation Kit quickly ligates sticky-end or blunt-ended DNA in less than 5 minutes at room temperature. The kit contains T4 DNA Ligase and specially formulated 5X Rapid Ligation Buffer, which is optimized for fast and efficient DNA ligation. For standard 1 hour ligation, rapid ligation efficiency is comparable to T4 DNA ligase. The ligation reaction mixture can be directly used for bacterial transformation with TransformAid Bacterial Transformation Kit or other conventional transformation procedures.

Product Advantages

• Fast—ligate sticky-end or blunt-end DNA in less than 5 minutes
• Convenient—reaction mixtures can be used directly on bacteria Transformation

Applications

• Conventional cloning experiments
• Blunt end cloning
• Library construction
• TA cloning

Includes

• T4 DNA Ligase
• 5X Quick Lig Buffer
• Water, nuclease-free
• Detailed protocol< br>
Note

Before electroporation, it must be purified by chloroform extraction or spin column (for example, by Thermo Scientific GeneJET PCR Purification Kit (K0701)) inactivates T4 DNA ligase.

Related products
Quick DNA ligation kit