PureLink™ Genomic Plant DNA Purification Kit can purify a variety of plant samples (e.g., spinach, tomato, soybean, etc.) in approximately 40 minutes , wheat, Arabidopsis and mushrooms) for efficient isolation of total DNA.
• Fast – purify total DNA from difficult plant samples in 1 hour
• Efficient – yield high quality DNA with minimal RNA contamination

Simple DNA from plant samples Purification
PureLink™ Genomic Plant DNA Purification Kit is suitable for isolating DNA from chloroplasts and a variety of plant tissues (Figure 1), such as alfalfa sprouts, sunflower sprouts, corn hulls, soybeans, Mushrooms, tomato leaves, wheatgrass and Arabidopsis leaves. The entire protocol relies on centrifugation on a spin column and does not require organic solvents. PureLink™ Genomic Plant DNA Purification Kit is designed to purify DNA from up to 100 mg of plant tissue.

Greatly improve the total DNA yield
High DNA yield can be achieved by using young plant samples such as leaves and freezing the samples in liquid nitrogen immediately after collection and very low levels of DNA degradation. Higher DNA yields can be obtained by eluting the total DNA in 2 aliquots (100 µL each), with the first elution yielding 65-80% DNA recovery and the second elution > 90%. Typically, A₂₆₀/A₂₈₀> of DNA isolated using the PureLink™ Genomic Plant DNA Purification Kit when diluting the sample with Tris-HCl (pH 7.5) 1.80, indicating that the DNA does not contain proteins that may interfere with downstream applications.

Isolated DNA can be used in a variety of downstream applications:
Total DNA purified from different plant tissues using the PureLink™ Genomic Plant DNA Purification Kit is suitable for a variety of applications, including PCR, restriction enzyme digestion, and Southern blotting.

Research use only. Not suitable for diagnostic procedures.