PureLink™ HiPure Expi Plasmid Macroprep Kit uses enhanced anion exchange resin in a single E. coli macroprep Higher-level isolation of transfection-grade plasmid DNA. PureLink™ HiPure Plasmid Macroprep Kits include vacuum filtration columns that allow bacterial filtration without centrifugation, allowing for extremely fast processing. The PureLink™ HiPure Expi Plasmid Macroprep Kit typically isolates 12 mg of high-quality, ultrapure plasmid DNA (low inherent endotoxin levels) from 2.5 L of bacterial culture.

PureLink™ HiPure Expi Plasmid Macroprep Kit benefits include:
•High yield—Isolate up to 14 mg of high-quality plasmid DNA in a single purification using 5 L of bacterial culture volume
br>• Purity—Low intrinsic endotoxin levels (typically 0.1–1.0 EU/µg) and A260/280 >1.8, making it ideal for transfection of mammalian cells
• Simple and ultra-fast protocol— Typical plasmid isolation is completed in as little as 90 minutes with a vacuum-assisted protocol

HiPure Expi Anion Exchange Chromatography for Plasmid Purification
PureLink™ HiPure Expi Plasmid Purification The kit uses a novel proprietary anion exchange resin to achieve a higher level of transfection-grade plasmid DNA purification. Enhanced resin combines high capacity, fast flow rates, high resolution and efficient endotoxin removal. Plasmid preparation is typically completed in less than 2 hours and has a purity equal to two CsCl gradients. PureLink™ HiPure Expi Plasmid Purification Kit enables a faster process for obtaining higher amounts of transfection-grade plasmid DNA.