PureLink™ Rapid Plasmid Miniprep Kit is designed for use with a silica membrane column to convert E. coli fromE. Quickly isolate sequencing-grade plasmid DNA. PureLink™ Rapid Plasmid Miniprep Kit Features:
•Fast — Ready-to-use plasmid DNA typically obtained in 30-45 minutes
• Flexible b> — Works with vacuum and centrifugation platforms
Reliable and cost-effective isolation of plasmid DNA
PureLink™ Rapid Plasmid Miniprep Kit uses PureLink™ spin columns in 30- Isolate high-quality plasmid DNA from 1–5 mL of culture in 45 minutes. These columns use a unique silica membrane to obtain up to 40 µg of sequencing-grade plasmid DNA. Cells are lysed using an alkaline/SDS procedure and the lysate is loaded onto a silica membrane column capable of selectively binding plasmid DNA. Use wash buffer to remove contaminants and TE buffer to elute plasmid DNA. The eluted DNA is suitable for all routine downstream applications.
Compatible with flexible formats
PureLink™ Rapid Plasmid Miniprep Kits can be used with a centrifuge or vacuum manifold to purify plasmid DNA. Typically, when samples are diluted with Tris-HCl (pH 7.5), A260/A280 > 1.80, indicating that the DNA does not contain proteins that may interfere with downstream applications.
Isolated DNA can be used in a variety of downstream applications
Plasmid DNA purified using the PureLink™ Rapid Plasmid Miniprep Kit is suitable for all common downstream applications, including bacterial and mammalian cells Transformation, DNA sequencing, restriction enzyme digestion, cloning, and PCR.
For Research Use Only. Not for use in diagnostic procedures.