pBAD TOPO™ TA Expression Kit is designed for one-step cloning and cloning of Taq amplified PCR products. Regulate prokaryotic expression. Once TOPO™ has cloned your PCR product, you can proceed directly to protein expression. Some convenient features of the pBAD-TOPO™ vector include:

• Linearized, topoisomerase I-activated vector for 5-minute cloning of Taq amplified PCR products
• araBAD promoter for tight control of expression in the E. coli V5 epitope tag for detection with anti-V5 antibodies
• C-terminal clustering Amino acid (6xHis) tag for purification using nickel chelating resin and detection with anti-His (C-terminal) antibody
• Enterokinase cleavage site for removal of N-terminal leader peptide