The Invitrogen™ pLenti6.3⁄V5™-TOPO™ Cloning Kit is part of our ViraPower™ HiPerform™ Lentiviral Expression System (Table of Contents No. K5310-00).

pLenti6.3⁄V5™-TOPO™ -TA Cloning Kit contains the TOPO™-adjusted ViraPower™ HiPerform™ lentiviral expression vector pLenti6.3⁄V5™-TOPO™ for use in splitting and non-split cells. Rapid PCR-based cloning and high-level expression of target genes in dividing mammalian cells. The pLenti6.3⁄V5™-TOPO™ vector is equipped with two key genetic elements that make it a HiPerform™ vector: the woodchuck post-transcriptional regulatory element (WPRE) from the HIV-1 integrase gene and the central polypurine pipe (cPPT) Sequences used to produce at least a 4-fold increase in protein expression compared to vectors lacking these elements.

Advantages
• Stable expression
• Long-term experiments
• Accurate titers of functional virus
• 5-minute high-efficiency TOPO™ PCR cloning, only Three simple steps required

Key Features
• HiPerform™ WPRE and cPPT elements
• CMV promoter
• V5 epitope tag (C-terminus)< br>• Blasticidin Selection

Kit includes
• lacZ vector as positive control, pLenti6.3⁄V5-GW⁄lacZ™
• Stbl3 ™ Competent Cells

For research use only. Not for therapeutic or diagnostic use.