ELISA Blocker blocking buffer is a protein buffer that can effectively block polypeptides after the initial coating step of the ELISA program. Styrene microplate binding sites. This ready-to-use buffer is optimized for use in sandwich ELISA procedures involving cytokines such as many Thermo Scientific Pierce ELISA kits.

ELISA blocking buffer characteristics:

•Specification:1L (enough for thirty 96-well plates)
•Application:< /b>Can be used as ELISA blocking buffer. This buffer is ready-to-use. No need to dilute.
•ELISA instructions for use: After incubation with the coating antibody, aspirate the coating antibody from the plate and add 300 μL ELISA Blocker blocking buffer to each well. Seal the membrane with sealing film and incubate at room temperature (22-25°C) for at least 1 hour. Aspirate the blocking buffer and proceed with the ELISA procedure or dry the plate for later use.
•Well plate drying: The well plate should be left open and dry at room temperature for at least 1 hour. To ensure ideal drying effect, the orifice plates cannot be stacked and the humidity should reach 30-40%. If the plates are stacked or the humidity is too high, the drying time should be extended. After drying is complete, each well plate is sealed in a foil bag using desiccant. When sealed with desiccant, plates can be stored at 2-8°C for up to 6 months.