Thermo Scientific phiX174 DNA is completely cut with appropriate Thermo Scientific restriction enzymes, purified and then redissolved in storage buffer . DNA fragments contain either blunt or sticky ends, depending on the type of restriction enzyme used to prepare the marker.

Thermo Scientific phiX174 DNA/BsuRI (HaeIII) markers can be used with T4 polynucleotide kinase Carry out radioactive labeling. Additionally, it is available via Klenow fragment or Klenow fragment (exo-) Fill-in reaction can be radioactive or non-radioactive labeled, see protocol in Resources.

Features

• Determine the size and approximate quantification of small molecule DNA fragments
• Obvious bands
• Provide matching solutions suitable for sample DNA Loading Dyes

Notes
• Bands of 310, 281, and 271 bp will form unusual patterns on polyacrylamide gels.
•The shortest fragments (skewed) are not visible in standard electrophoresis.
• Predict fragment lengths by computer analysis of corresponding DNA sequences.