Thermo Scientific RiboRuler High Range RNA Molecular Weight Standard (200-6000 bases) is composed of 8 chromatographically purified single-stranded A mixture of RNA transcripts (200-6000 bp long) generated from a specific template containing a pTZ19R multisite adapter fragment and a Lambda phage fragment.

RiboRuler High Range RNA Molecular Weight Standards are supplied as 1 mM EDTA, pH 6.0.

RiboRuler High Range RNA Molecular Weight Standards are composed of chromatographically purified transcripts, which makes them free of NTPs and RNA degradation products. The concentration of each RNA transcript was determined spectrophotometrically. To minimize the possibility of contamination during use, aliquot molecular weight standards into smaller volumes.

RiboRuler High Range RNA Molecular Weight Standards are terminated using T4 Polynucleotide Kinase Ideal for labeling (see protocol in Resources) and also ideal for Northern blotting.

Product Advantages

• Clear bands with uniform intensity
• Easy to remember band size and number
• Molecular weight standard band Predetermined concentrations facilitate approximate RNA quantification
• Comes with 2X RNA loading dye

Application

• On native or denaturing gels Perform RNA sizing and quantification
• Northern blot