Lipofectamine Stem Cell Transfection Reagent is optimized to achieve higher efficiency with less early differentiation in a variety of stem cells. Capable of co-delivering DNA, RNA and Cas9 ribonucleoprotein (RNP) complexes. Lipofectamine Stem Cell Reagent is compatible with a variety of media systems, including feeder-free media systems, helping to support and simplify stem cell culture workflows. Researchers can achieve transfection efficiencies of up to 80% or higher in pluripotent stem cells (PSCs) and neural stem cells (NSCs), and up to 45% in mesenchymal stem cells (MSCs).

Transfecting stem cells without inhibiting cell viability and cell growth can be challenging due to the sensitive nature of these cells. Lipofectamine Stem Cell Reagent is a low toxicity reagent that requires a small amount of nucleic acid to allow your stem cells to stay healthy and continue to proliferate without inducing differentiation. Lipofectamine Stem Cell Reagent:

Excellent Performance—3X more efficient than other reagents in iPSCs, hESCs, NSCs, and MSCs
Universal— DNA, RNA, Cas9 protein and co-transfection using one reagent that is
gentle on cells—sustains cell proliferation and does not induce cell differentiation
flexible— Can transfect adherent or suspension cells—an alternative to electroporation

Efficient gene editing in stem cells
Gene editing applications often require the ability to co-deliver DNA , RNA and/or proteins into cells. Lipofectamine Stem Cell Transfection Reagent is designed to co-deliver CRISPR/Cas9 complexes into human stem cells for efficient gene editing. This reagent can be used to transfect large DNA plasmids, such as monolithic CRISPR/Cas9 vectors, and to co-deliver a range of RNAs, such as large Cas9 mRNA or unidirectional (sg) RNA with tracr/crRNA complexes, as well as proteins, such as with tracr/ crRNA or sgRNA binds to Cas9 protein.

Low-toxic alternatives to electroporation
Transfecting stem cells by electroporation methods can be challenging and complex. Electroporation protocols recommend the use of single-cell suspension culture to ensure higher efficiency and lower toxicity. Using a reverse transfection protocol, Lipofectamine Stem Cell Transfection Reagent was demonstrated to be an effective and less toxic alternative to stem cell electroporation. This technology requires only a small amount of nucleic acid to achieve high transfection efficiency, equivalent to electroporation, while supporting continued proliferation without inducing differentiation.