The Phospha-Light™ Assay System is a chemiluminescent reporter assay for sensitive detection of secreted forms of reporter proteins Human placental alkaline phosphatase.

• Secreted reporter assays allow analysis without destroying valuable cells.
• The wide dynamic range of the alkaline phosphatase assay allows users to accurately measure enzyme levels from femtogram to nanogram levels.
• This detection technology is 100 to 1,000 times more sensitive than the isotopic/nonisotopic detection of chloramphenicol acetyltransferase (CAT) or the colorimetric/fluorescent detection of b-galactosidase. times, its sensitivity is higher than that of other competing detection technologies.
• Highly sensitive assay with wide dynamic range detects both high and low levels of reporter genes without the need to dilute large amounts of sample.
• Non-radioactive reporter gene assay kits eliminate concerns about the use of radioactive isotopes.
•The test can be completed in approximately 1 hour, enabling rapid test turnaround.

Monitor expression over time
The Phospha-Light™ Assay System provides sensitive detection of secreted alkaline phosphatase (SEAP) reporter proteins in cell culture media. The SEAP gene product is a truncated form of human placental alkaline phosphatase. Secreted reporter proteins do not require the preparation of cell extracts. Cell populations can be monitored over time and kept intact for further experiments.

Patented reagents for ultra-sensitive detection
The Phospha-Light™ detection system is one of the simpler and faster ways to optimize transfection efficiency. The patented reagent combination is the key to the high sensitivity of the Phospha-Light™ test. The system includes CSPD™ high performance alkaline phosphatase substrate, Emerald™ chemiluminescence enhancer and a specific buffer system designed to inhibit non-placental endogenous phosphatase activity. The persistent glow of the chemiluminescence signal can be measured using a photometer without the need for a sample injector.

Application
. The Phospha-Light™ Reporter Assay System has been widely used as a reporter assay to measure gene expression in established cell lines and transfected primary cells, including as a gene knockdown⁄RNA interference readout method. Phospha-Light reporter assays are used for a wide range of viral functional assays, including viral gene expression experiments, viral replication, viral fusogenicity, virus neutralization and virus-mediated cell-cell fusion, and viral infectivity. The use of SEAP reporter proteins is very useful for in vivo detection of reporter genes by detecting the serum of transgenic, transfected, or viral vector-infected animals. The Phospha-Light reporter gene assay system has been used to measure SEAP levels in the serum of transgenic or transfected whole animals, including mouse, rat, marmoset, monkey, and pig serum, and chicken egg allantoic fluid. Recently, mouse SEAP protein (mSEAP) was developed to improve the stability of SEAP in transgenic mice, and the Phospha-Light™ system has been used for sensitive detection of mSEAP. For these and additional references, see the bibliography in the Literature⁄Resources section.

Research use only. Not intended for diagnostic use.