Thermo Fisher TS-38830 Each BSTFA Silanization reagent
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Improve GC analysis with Thermo Scientific™ BSTFA Silanization Reagent. The highly volatile by-products mono(trimethylsilyl)trifluoroacetamide (BSTFA) and trifluoroacetamide elute with the solvent front, ensuring excellent chromatographic separation. This silanization reagent can react with a series of polar compounds and is particularly suitable for GC analysis of certain low-boiling point TMS amino acids and TMS tricarboxylic acid cycle acids.

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BSTFA is a powerful trimethylsilane donor, comparable in strength to the non-fluorinated analog BSA [N,O-bis(trimethylsilyl)acetamide]. BSTFA can replace unstable hydrogen atoms in a wide range of polar compounds with -Si(CH3)3 groups. This physical property is particularly useful for gas chromatographic analysis of some lower boiling TMS amino acids and the TMS tricarboxylic acid cycle.
  • Compared with the non-fluorinated by-products of BSA, its reaction by-products mono(trimethylsilane) trifluoroacetamide and trifluoroacetamide are more volatile.
  • The increased volatility allows TMS to derivatize smaller molecules by derivatizing the by-products of eluting BSA.
For scientific research use only. Not for use in diagnostic procedures.
References:
  1. Her, GR, et al.(1985).Anal .Biochem.151, 292-298.
  2. Shanchun, J., et al.(1994).Org.Geochem.21, 415- 422.