CyQUANT MTT Cell Viability Assay determines mammalian cell viability using the proven and widely used MTT reagent. The redox potential in active mammalian cells reduces MTT to an intensely staining formazan product. After solubilization, the absorbance of formazan can be measured using a microplate absorbance reader. The CyQUANT MTT Cell Viability Assay Kit is a complete and optimized kit that provides all the reagents needed to detect mammalian cell viability. The kit provides sufficient material for ∼1000 assays.

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Features of the CyQUANT MTT Cell Viability Assay Kit include:
• Complete and optimized kit for mammalian viability Colorimetric detection of cells
•Utilizes the proven MTT reagent as a reporter molecule for cellular redox potential
•Ability to perform 1,000 tests using 96-well microplates

Measuring changes in cell viability is an assessment Fundamental methods for cellular health, measuring genotoxicity, and evaluating anticancer drugs. A variety of methods are available for such assays, but procedures using colorimetric indicators provide a rapid and cost-effective method for determining changes in mammalian cell viability. Among various colorimetric cell viability assay kits, the MTT assay kit is a mature and commonly used assay kit.

Redox potential in living mammalian cells results in water-soluble MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide ) is converted into insoluble formazan products. The concentration of the colorimetric probe is determined from optical density measurements at a wavelength of 570 nm after solubilization of formazan using the supplied SDS (sodium dodecyl sulfate) reagent.

CyQUANT MTT Cell Viability Assay Kit provides a simple method to measure mammalian cell viability using a standard microplate absorbance reader. Simply prepare the MTT reagent, add it to the cells, dissolve the formazan produced, and measure the optical density using a standard microplate reader. The CyQUANT MTT Cell Viability Assay Kit provides the necessary materials to perform 1,000 tests using a standard 96-well microplate.