Minimum Essential Medium (MEM) is a commonly used cell culture medium. MEM can be used to culture a variety of suspended and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes wait. Gibco™ MEM Modified Medium is available in a variety of components for a wide range of cell culture applications. Use
Medium Selection Tool Find the right recipe.
The improved form of this MEM is as follows:
Includes | Does not contain | < /tr>
• NEAA | • L-Glutamine |
Provide complete
Formulation.
Gibco™ MEM was developed by Harry Eagle based on his earlier Basic Eagle Medium (BME) formulation. Many other modifications of MEM have since been made, including Glasgow's MEM, MEM alpha, DMEM, and Temin's modified medium. MEM contains Earle's Balanced Salts for CO2
incubators, or Hanks' Balanced Salts for CO2-free incubators. This product is made with Earle's salt.
cGMP Manufacturing and Quality SystemsGibco™ MEM is manufactured in a cGMP compliant facility in Grand Island, New York. The factory is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard. To ensure a stable supply chain, we also offer the same Gibco™ MEM product (10370-047) manufactured in our Scottish factory. The facility is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard.
MEM does not contain proteins, lipids or growth factors. Therefore, MEM needs to be supplemented with nutrients, usually 10%
Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO
2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.