Minimum Essential Medium (MEM) is a commonly used cell culture medium. MEM can be used to culture a variety of suspended and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes wait. Gibco™ MEM Modified Medium is available in a variety of components for a wide range of cell culture applications. Use
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The improved form of this MEM is as follows:
Includes | Does not contain | < /tr>
• L-Glutamine | • HEPES: |
• Gentamicin | • Phenol Red |
• Proline | • Insulin |
• Sodium Pyruvate | |
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Provide complete
formula .
Gibco™ Richter's MEM is an improved formula of Eagle MEM, which contains iron, zinc, putrescine, fatty acids and gentamicin. Richter's is modified to also contain very high concentrations of choline chloride and myo-inositol. This product is made from Earle's Balanced Salt.
Product UseResearch Use Only: Not for diagnostic or therapeutic use in animals or humans.
cGMP ManufacturingGibco™ MEM is manufactured in a cGMP compliant facility in Grand Island, New York. The facility is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard.
Gibco™ MEM Medium does not contain proteins, lipids or growth factors. Therefore, MEM needs to be supplemented with nutrients, usually 10%
Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO
2 environment to maintain physiological pH.
For Research Use Only. Not for use in diagnostic procedures.