Thermo Fisher 11320033 500 mL DMEM/F-12
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11320033 500mLDMEM/F-12

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DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium , can be used to support the growth of many different types of mammalian cells. Cells that have been successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We provide a variety of DMEM/F-12 modified media for a wide range of cell culture applications. Use Medium Selection Tool Find the right recipe.

This DMEM/F-12 modification is as follows:
Contains Does not contain
•L-Glutamine•HEPES:
• Phenol Red

Available in fullFormulation.

Use DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. The formula contains DMEM's high concentrations of glucose, amino acids, vitamins and various components of F-12. DMEM/F-12 contains no proteins, lipids or growth factors. Therefore, DMEM/F-12 may require nutritional supplementation, typically 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires an environment of 5–10% CO2 to maintain physiological pH.

cGMP Manufacturing and Quality Systems
DMEM/F-12 is manufactured in a cGMP compliant facility in Grand Island, New York. The factory is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard. To ensure supply chain continuity, we also offer an equivalent DMEM/F-12 product (11320-074) manufactured at our Scottish facility. The latter is also a medical device manufacturer registered with the FDA and complies with the ISO 13485 standard.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.