Thermo Scientific Phusion Blood Direct PCR Kit was developed to amplify DNA from whole blood. No separate DNA purification step is required before PCR. Modified Phusion Hot Start II High-Fidelity DNA Polymerase exhibits high tolerance to PCR inhibitors found in blood, retaining polymerase activity even in samples containing 40% whole blood.
This DNA polymerase exhibits exceptional accuracy in proofreading and makes the Phusion Blood Direct PCR Kit one of the best choices for demanding applications such as cloning. The versatility of the Phusion Blood Direct PCR Kit also allows the analysis of blood samples from different species, as well as the analysis of blood samples using different anticoagulants or storage conditions.
Product Benefits:
• No need for time-consuming and expensive DNA purification steps
• Simple protocol requires minimal hands-on time
• Extremely short PCR protocol time
• Improved Phusion Hot Start II High-Fidelity DNA Polymerase Can Achieve Extremely High Specific Product Yields
The optimal annealing temperature of Phusion DNA Polymerase may be different from that of
Taq-based polymerase Significantly different.
For best results, first use our Tm CalculatorAccurately calculate your Tm. For Research Use Only. Not for use in diagnostic procedures.