Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F-12) is a widely used basal culture base that can be used to support the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK cells, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. For a wide range of cell culture applications, we offer a variety of Gibco™ DMEM/F-12 modified media. Use Medium Selection Tool Find the right recipe.
Full Formulation.

Gibco™ DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12 Medium. This formula combines the high concentration of glucose, amino acids and vitamins in DMEM with various components of F-12.

cGMP Manufacturing and Quality Systems
Gibco™ DMEM/F-12 is manufactured in a cGMP compliant facility in Paisley, Scotland, UK. The facility is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard.

DMEM/F-12 contains no proteins, lipids or growth factors. Therefore, DMEM/F-12 may require nutritional supplementation, typically 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO₂ environment to maintain physiological pH.