Thermo Fisher F631XL 2000 x 50 μL reactions Phusion Plus PCRmaster mix
$1187.29 / Parcel$2473.38 Min.order:1
Brand Sales
F631XL 2000 x 50 μL reactionsPhusion Plus PCR Master Mix

Support Payment Term:

HSBC Hong Kong

paypal

Alibaba Pay

Western Union

USD

EUR

GBP

SGD

HKD

CNH

CAD

MXN

BRL

JPY

THB

MOP

AUD

NZD

PLN

CZK

HUF

RON

CHF

SEK

NOK

DKK

TRY

AED

SAR

ILS

ZAR

$0.00

Quantity

Discover similar items

Page 1 of 2

Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mix containing Phusion Plus DNA Polymer Enzymes, buffers, magnesium chloride and dNTPs. Master mix formats simplify reaction preparation with minimal pipetting steps while retaining all the properties of individual formats.

High fidelity — Sequence accuracy >100 times higher than Taq polymerase
Easy to set up — Use the same sequence in PCR Annealing temperature 60°C
Higher specificity — Hot start modification reduces non-specific amplification and primer degradation
Bench stability — The prepared reaction system is stable for 24 hours and can be automated
High inhibitor tolerance — suitable for DNA with poor purity

Phusion Plus DNA polymerase is a Hot-start high-fidelity DNA polymerase using protein fusion technology and universal primer annealing capabilities. It is a Pyrococcus -like proofreading DNA polymerase fused to a processivity-enhancing domain, allowing Phusion Plus PCR Master Mix to generate PCR sequences with high accuracy, sensitivity, and inhibitor tolerance. Its novel reaction buffer contains a proprietary additive that stabilizes the primer/template duplex during the 60°C annealing step. This unique buffer formulation improves PCR specificity by eliminating the need to optimize annealing temperatures for each primer pair individually, helping to save time and avoid human error in PCR runs. In addition, with Phusion Plus PCR Master Mix and its universal annealing function, different PCR reactions can be cycled simultaneously using the same protocol.

Phusion Plus PCR Master Mix is inactivated at room temperature due to a hot-start mechanism mediated by the parent molecule, allowing for preparation and storage of PCR reactions prepared at room temperature. Enzyme activity is not fully activated until the predenaturation step.

Applications
• High fidelity PCR
• Cloning
• Generating sequencing templates
• High GC content PCR
• Long fragment PCR (long up to 20 kb)
• Mutation
• High-throughput PCR

Learn about Phusion Plus polymerase products More information›
For Research Use Only. Not for use in diagnostic procedures.