DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium , can be used to support the growth of many different types of mammalian cells. Cells that have been successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We provide a variety of DMEM/F-12 modified media for a wide range of cell culture applications. Use Medium Selection Tool Find the right recipe.
Full Formulation.

Use DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. The formula contains DMEM's high concentrations of glucose, amino acids, vitamins and various components of F-12. DMEM/F-12 contains no proteins, lipids or growth factors. Therefore, DMEM/F-12 may require nutritional supplementation, typically 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

cGMP Production and Quality System
To ensure the stability of the supply chain, we simultaneously produce DMEM/F- 12. Both factories comply with cGMP production requirements, are ISO 13485 certified, and are FDA-registered medical device manufacturers.