MEM α (Minimum Essential Medium α) is widely used in mammalian cell culture and selection of transfected DHFR-negative cells. MEM α can be used on a variety of suspended and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. For a wide range of cell culture applications, a variety of MEM alpha modified media are provided. Use Medium Selection Tool Find the right recipe.
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Using MEM α
MEM α is a modified version of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B₁₂, biotin and ascorbic acid. MEM α is also available in a nucleoside-free version for use as a selection medium for DG44 and other DHFR-negative cells. Contains GlutaMAX™ Supplemented MEM alpha medium comes in an easy-to-use packaged format and significantly reduces the accumulation of toxic ammonia and improves cell viability and growth. This product is made from Earle's Balanced Salt. MEM alpha contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, usually 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

cGMP Manufacturing and Quality Systems
MEM α is manufactured in a cGMP compliant facility in Grand Island, New York. The factory is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard. To ensure a stable supply chain, we also offer the same MEM α product (32561-094) manufactured in our Scottish factory. The factory is also an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard.