Waymouth Medium is a chemically defined medium developed by Charity Waymouth and originally formulated for use in a serum-free environment The nutrition, metabolism and growth of the mouse cell line L subline NCTC clone 929 (L929 cells) were studied. Waymouth medium is also used for the culture of whole organs and various cell types, such as cancer cell lines.
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Intended Use of Product
In vitro diagnostics. Caution: Not for therapeutic use in humans or animals. Use other than as indicated on the product may violate local regulations.

Dual on-site cGMP manufacturing
Gibco™ Waymouth Medium is manufactured in a cGMP compliant facility in Grand Island, New York. The factory is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard. To ensure a stable supply chain, we also offer the same Gibco™ Waymouth product (31220-023) manufactured in our Scottish factory. The latter is also a medical device manufacturer registered with the FDA and complies with the ISO 13485 standard. Waymouth medium does not contain proteins, lipids, or growth factors. Certain cell types may require the addition of Fetal bovine serum (FBS). Waymouth uses a sodium bicarbonate buffer system (2.24 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.