Thermo Fisher 10533073 3 x 500 unitsterminal deoxynucleotidyl transferase, recombination
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10533073 3 x 500 units terminal deoxynucleotidyl transferase, recombinant

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Terminal deoxynucleotidyl transferase, recombinant (rTdT) is a DNA polymerase that catalyzes the transfer of 3fi hydroxyl groups to DNA A reaction in which deoxyribonucleotides are added to the terminal ends. Provide TdT technical bulletins.

Applications:
Homopolymer tailing of vectors and inserts for cloning. Oligonucleotides labeled with biotin (1,2), 32P or 35S (3), or in cells undergoing apoptosis (TUNEL) (4, 5) Medium.

Source:
Purified from calf thymus TdT E. coli clone.

Performance and quality testing:
Endonuclease, 3´ and 5´ exodeoxyribonuclease and measured incorporation levels.

Unit definition:
One unit incorporates 1 nmoL dATP into an acidic precipitable material (within 1 h at 37°C using d(pA)50 As a primer.

Hazard warning:
Toxic; reaction buffer contains potassium cacodylate. Also contains highly toxic chemical: cobalt chloride.
See MSDS. .

Unit reaction conditions:
0.2 M potassium cacodylate (pH 7.2), 10 mM MgO4 C4 H6, 1 mM 2-mercaptoethanol, 0.5 mg/ml BSA,
100 flM d(pA)50, 1 mM [3
sup>H]dATP and enzyme (0.15 mL, 1 h, at 37°C)
For Research Use Only. Not for use in diagnostic procedures. .