5'

A

C

C

T

G

C

N4

↓

3'

3'

T

G

G

A

C

G

N8

↑

5'

Thermo Scientific FastDigest BveI restriction enzyme recognizes ACCTGC(4/ 8)^ site and cleaves optimally within 5–15 minutes at 37°C using Universal FastDigest Buffer. Iso-cleavage enzymes: BspMI, Acc36I, BfuAI.

Thermo Scientific FastDigest BveI is one of a series of high-end fast restriction enzymes that are 100% active in both Universal FastDigest Buffer and FastDigest Green Reaction Buffer.

Universal buffer turns green in 5–15 minutes, eliminating the need for buffer changes or subsequent DNA purification steps. For a table of enzymatic activities, heat inactivation, and incubation times for this and other FastDigest restriction enzymes, see Reaction conditions for FastDigest enzyme< /a>. DNA modifying enzymes (such as Klenow fragment, T4 DNA Ligase, alkaline phosphatase and T4 DNA Polymerase) are 100% active in FastDigest buffer. Therefore, enzymes used in downstream applications can be added directly to the FastDigest reaction mixture.

For convenience, FastDigest Green Buffer includes 1 density reagent and 2 tracking dyes for loading digestion reaction products directly onto the gel.

Shorter incubation times and optimized universal FastDigest buffer composition eliminate star activity effects.

Features

•100% activity of all FastDigest enzymes in universal buffers
•100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Load directly onto gel
• No star activity
• 176 FastDigest enzymes available

Applications< /b>

•Molecular cloning
•Restriction site mapping
•Genotyping
•Southern blot
•Restriction fragment length polymorphism (RFLP)
• SNP Analysis

Remarks: To achieve efficient cleavage, at least two copies of the FastDigest BveI recognition site are required. Adding 0.5 μM oligonucleotide containing FastDigest BveI recognition sequence to the reaction mixture can significantly improve the digestion efficiency of plasmid DNA, especially DNA with a single FastDigest BveI site. FastDigest BveI still has difficulty digesting some substrates completely.

FastDigest Green Buffer provides the same high performance as colorless FastDigest Buffer in DNA digestion reactions and downstream applications. For applications requiring product analysis by fluorescence excitation (eg determination of concentration under UV light), the use of colorless FastDigest buffer is recommended.

See product specifications for information on methylation sensitivity.