Dynabeads™ mouse pan T (Thy 1.2) is a magnetic bead that provides a robust, versatile tool for direct Isolate or remove mouse T cells from any sample, including tissue digests such as spleen or lymph nodes, whole blood, bone marrow, and mononuclear cells (MNC).
• Rapid isolation of mouse T cells from any sample – no columns required
• Positive isolation for molecular downstream detection or efficient removal of mouse T cells
Recovered cells have Excellent yield, purity and viability Dynabeads™ Mouse pan T (Thy 1.2) are homogeneous, superparamagnetic microbeads (4.5 µm diameter) that easily isolate small cells from spleen or lymph nodes Murine T cells, but any single cell suspension may be used. Dynabeads™ mouse pan T beads are coated with a primary monoclonal antibody specific for the Thy 1.2 membrane antigen expressed on peripheral T cells, thymocytes, and intraepithelial T lymphocytes of all common mouse strains. Add Dynabeads™ mouse pan T to the culture tube, which binds T cells during a short incubation period, then use
Magnet Stand Separates bead-bound cells from unbound cells. This rapid and gentle separation method does not require the use of chromatography columns and helps ensure high purity, recovery, and viability of isolated T cells.
Suitable for removal and positive isolation of downstream molecular detection Dynabeads™ mouse pan T (Thy 1.2) can be used with viscous samples thanks to the powerful magnetic stand for separation , such as single cell suspensions from tissue, whole blood, and bone marrow, and can effectively remove mouse T cells in approximately 30 minutes. Dynabeads™ mouse pan T also allows efficient recovery of highly pure viable cells when used for positive isolation for downstream research; for example, cells are lysed but remain attached to the beads and further purified nucleic acids or proteins. Note that these beads do not release intact cells, so if you want to isolate mouse T cells for cell-based applications, or need to examine samples using flow cytometry, you should use
Dynabeads™ FlowComp Mouse Pan T (CD90.2) (for bead-free cells).
Please download from our company's
Mouse T cell isolation products series.
For scientific research use only. Not for therapeutic or diagnostic use in humans or animals.
For Research Use Only. Not for use in diagnostic procedures.