This gentle, easy-to-use kit isolates mouse CD4+ T cells that are compatible with flow cytometry. The magnetic beads are released and removed from the cell sample immediately after positive isolation. In this way, you avoid exposing cells to potentially cytotoxic or immunogenic foreign substances (such as iron oxides or dextran), which are potential issues when using biodegradable particles. Additionally, your cells will not be exposed to stress conducted through the column.

Achieve >85% purity level and >85% viability of mouse CD4+ cells.

Advantages:

• Results are independent of separation method
• Gentle test tube method - no column required
• High purity, high recovery and viability
• Bead-free cells - compatible with flow cytometry analysis
• Live bead-free CD4+ T cells, ready for use in any cell assay

Isolated cells have Expected properties of memory and naïve CD4+ T cells with high proliferative capacity. Saving you time and money and increasing confidence in your results.

Starting sample: Spleen or lymph node cells