The improved form of this MEM is as follows:

< /tr>

Includes	Does not contain
• Phenol Red	• L-Glutamine
• Earle's Salt	• HEPES:
	• Sodium bicarbonate

Provide complete formula .

Gibco™ MEM was developed by Harry Eagle based on his earlier Basic Eagle Medium (BME) formulation. Many other modifications of MEM have since been made, including Glasgow's MEM, MEM alpha, DMEM, and Temin's modified medium. To autoclave this medium, choline tartrate was used instead of choline chloride, phenol red was reduced and succinic acid was added to prevent precipitation at low pH. This MEM formulation contains Earle's salt for use in CO₂ incubators. This product is made from Earle's salt.

Dual on-site cGMP manufacturing
For supply chain continuity, we manufacture Gibco™ MEM at two separate facilities in Grand Island, New York and Scotland, UK. Both factories comply with cGMP production requirements, are ISO 13485 certified, and are FDA-registered medical device manufacturers.

MEM does not contain proteins, lipids or growth factors. Therefore, MEM needs to be supplemented with nutrients, usually 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

This autoclavable form of Gibco™ cell culture medium requires pH adjustment to 4.1 - 4.2 prior to autoclaving, followed by supplementation with sodium bicarbonate and L-glutamine to achieve final pH Value adjusted to 7.2 – 7.4 (for details, see Proposal).