CTS™ (Cell Therapy System) Synth-a-Freeze™ Medium is a chemically defined, protein-free Liquid cryopreservation medium intended for freezing and storage of various mammalian cell types, including stem cells. Synth-a-Freeze™ CTS™ Medium is optimized to contain no antibiotics, antifungals, hormones, growth factors, serum or proteins and uses HEPES and sodium bicarbonate as a buffer system.

Suitable for cryopreservation of a variety of primary cells and stem cells

• Compatible with various freezing protocols, and the thawed culture medium can be stored stably at 4°C, thus saving time and energy
• Prepare for clinical research with this cGmP-compliant, chemically defined, protein-free formula

Cryopreserve multiple cell types
Cells Therapeutic System Synth-a-Freeze™ CTS™ Medium has performance comparable to standard serum-containing cryopreservation media and is suitable for the cryopreservation of a variety of cell types, including human embryonic stem cells, neural stem cells (Reference 1), mesenchymal stem cells, Mesenchymal stem cells as well as keratinocytes (Ref. 2). Synth-a-Freeze™ medium is not recommended for cryopreservation of human epidermal melanocytes.

Easily integrates into your current freezing protocol
Synth-a-Freeze™ CTS™ Medium can be used in any standard freezing protocol. Simply add thawed Cell Therapy System Synth-a-Freeze™ CTS™ Medium to the isolated and pelleted cells, aliquot and freeze. In order to obtain ideal results, it is recommended to use a programmable cooling device.

Simplifying the transition to clinical research
The Gibco™ Cell Therapy System (CTS™) brand offers a broad range of products for a variety of cell therapy applications, including culture media, reagents , growth factors, enzymes, cell selection/amplification reagents, and equipment. Gibco™ Cell Therapy System (CTS™) products offer the following features:
• Reduce the burden of reagent validation
• Include appropriate Certificates of Analysis and Certificate of Origin
• Convenient access to Drug Master Files ( DMF)

Suitable for research use or the production of cell, gene or tissue related products.

Note: Not for direct administration to humans or animals.

References:
• S. Shin and M. Vemuri.Culture and Differentiation of Human Neural Stem Cells.LCDoering (ed).Protocols for Neural Cell Culture.Springer Protocol Handbooks.
• S. Lemaire, K. Kosowska-Shick, P. Appelbaum, G. Verween, P. Tulkens, and F. Van Bambeke. Cellular Pharmacodynamics of the Novel Biaryloxazolidinone Radezolid: Studies with Infected Phagocytic and Nonphagocytic cells, Using Staphylococcus aureus, Staphylococcus epidermidis, Listeria monocytogenes, and Legionella pneumophila. Antimicrob Agents Chemother 54(6) 2010: 2549-2559.