Gateway BP Clonase® enzyme contains both Int (Integrase) and IHF (Integrating Host Factor) proteins, for clones containing attB site) and the donor vector (containing attP site), resulting in entry clones. BP Clonase® II enzyme format is a single mixture of enzyme and buffer that ensures enzyme stability and allows for convenient setup of 10μL reactions with fewer pipetting steps, which maximizes reconstitution efficiency and ease of pipetting Minimize steps as much as possible. The initial form of BP Clonase enzyme is a stand-alone enzyme mix and reaction buffer provided in separate tubes.