199 Medium was originally developed for nutritional studies of chicken embryo fibroblasts. It has broad species applicability and is particularly suitable for the culture of non-transformed cells. 199 Medium is widely used in virology, vaccine production, and for the in vitro culture of primary explants of mouse pancreatic epithelium and rat lens tissue. We offer a variety of Gibco™ 199 modified media for a wide range of cell culture applications. Use Medium Selection Tool Find the right recipe.
Full formulation is available.

Compared with other basal media, 199 medium contains unique ingredients, including adenine, adenosine, hypoxanthine, thymine and other vitamins. 199 Medium contains Earle's salts for use in CO₂ incubators, or Hanks' salts for use in CO₂-free incubators.

Intended Use of Product
For use in in vitro diagnostics. Note: Not for therapeutic use in humans or animals. Unintended use may violate local laws.

cGMP Manufacturing and Quality System
Gibco™ 199 Medium is manufactured in a cGMP compliant facility in Grand Island, New York. The factory is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard. To ensure a stable supply chain, we also offer the same Gibco™ 199 media product (21180-021) manufactured in our Scottish facility. The facility is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard.

199 medium does not contain proteins, lipids or growth factors. 199 The culture medium uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

The concentrated form of Gibco™ 199 media requires the addition of sodium bicarbonate, pH adjustment, and filtration (for details, see Protocol).