Invitrogen Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines ultra-high fidelity with innovative buffers Together, they can maximize the annealing temperature of PCR universal primers. It is ideal for cloning, mutagenesis, and other experimental applications requiring excellent sequence accuracy.

Platinum SuperFi II DNA Polymerase features include:
•Excellent>300X Taq fidelity
•Universal primer annealing temperature of 60°C
•Excellent specificity , sensitivity and yield
• Robustly amplifies difficult-to-amplify targets (e.g. targets with poor purity, targets with GC content ˃65%, targets requiring long-range PCR)

Platinum SuperFi II DNA polymerase is an engineered enzyme with high processivity and enhanced resistance to PCR inhibitors. It also enables rapid cycling protocols and amplification of long targets (up to 20 kb). Platinum hot-start technology uses proprietary antibodies that inhibit enzyme activity prior to the PCR denaturation step, preventing non-specific amplification and primer degradation. This technology also enables reaction setup at room temperature, improving sensitivity and yield.

Due to the unique composition of SuperFi II PCR buffer, most primer pairs designed according to general design rules have an annealing temperature of 60°C. Isostabilizing molecules in the buffer increase primer-template dual stability during the annealing step and enhance specificity without the need to optimize the annealing temperature for each primer pair. Using Platinum SuperFi II DNA Polymerase, different PCR assays can be co-cycled using the same protocol using universal primer annealing temperatures and extension steps selected for the longest fragment to be amplified.

Applications for Platinum SuperFi II DNA Polymerase:
• High-fidelity PCR
• Cloning and subcloning
• Site-directed mutagenesis
• Amplification of GC-rich templates
• Generate templates for sequencing
• High-throughput PCR
• Amplify samples of poor purity
• Long fragment PCR
• Fast PCR

For improved For convenience, we offer Platinum SuperFi II Master Mix and Platinum SuperFi II DNA Polymerase ready-to-use type mix and SuperFi II PCR buffer and dNTPs, thus reducing the number of pipetting steps during PCR reaction setup. You can also use Platinum SuperFi II Green PCR Master Mix, which also contains a density reagent and two A tracking dye is used to load PCR products directly onto the gel, further simplifying the PCR workflow from setup to final analysis.

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