Gateway™ technology enables rapid cloning of one or more genes into virtually any protein expression system. Once you have an entry clone, you can recombine your gene of interest into a variety of expression vectors suitable for Gateway™ technology. PCR products are directionally cloned with typically >99% efficiency. The pDONR™ 221 vector features pUC replication region sequences for high plasmid yields and a convenient universal M13 sequencing site.