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5'		R	C	A	T	G ↓	Y		3'
3'			Y ↑	G	T	A	C	R		5'

Thermo Scientific XceI (NspI) restriction enzyme recognizes the RCATG^Y site and cleaves well in Tango buffer at 37°C. See Reaction Conditions for Restriction Enzymes table to learn about the enzymatic activity of this enzyme and other restriction enzymes , double enzyme digestion conditions and heat inactivation. Note: FastDigest enzyme is also available for rapid DNA digestion. Iso-cleavage enzymes: BstNSI, NspI.

Thermo Scientific Conventional Restriction Endonucleases are a large line of high-quality restriction enzymes optimized to function in one buffer of a five-buffer system. In addition, a universal Tango buffer is provided to facilitate double enzyme digestion. All enzymes demonstrated 100% activity under recommended buffers and reaction conditions. To ensure consistent performance, Thermo Scientific Restriction Enzyme Reaction Buffers contain premixed BSA, which enhances the stability of multiple enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—strict quality control and industry-leading production processes
• Convenient color-coded five-buffer system
•Includes universal Tango buffer for double digestion
•Pre-mixed BSA in reaction buffer
•Multiple restriction endonuclease specificities available

< b>Applications

•Molecular cloning
•Restriction site mapping
•Genotyping
•Southern blot
•Restriction fragment length polymorphism ( RFLP)
•SNP

Note:For methylation sensitivity, see product specifications.