Ambion™ RNase Cocktail™ is available in RNase A (500 U⁄mL) and RNase T1 (20,000 U⁄mL) A mixture of highly pure ribonucleases that are free of DNase and cleavage activity. Use RNase Cocktail in all situations where RNA degradation is appropriate (ie, plasmid minipreps and ribonuclease protection assays). For maximum convenience, RNase Cocktail is supplied as 50% glycerol. Digestion of RNA using RNase A alone leaves RNA fragments large enough to be visible on an agarose gel and precipitable in ethanol. Cleavage is performed using RNase A after C and U residues and RNase T1 after G residues. Thus, a mixture of the two enzymes resulted in a reduction in RNA fragment size compared to either enzyme alone.

Quality Control
RNase Cocktail Enzyme Mix is rigorously tested for contaminating non-specific endonuclease, exonuclease and protease activity. Functionality was determined in an RNase protection assay.

Unit definition:
RNase A: One unit of RNase A is the amount of enzyme required to produce an increment of 0.0146 absorbance units/minute at 286 nm in a volume of 1 mL. . Unit assay conditions: 100 mM Tris-acetate (pH 6.5), 1 mM EDTA and 1 mM cyclic 2',3'-CMP.

RNase T1: 100 units of RNase T1 is the amount of enzyme required to produce an absorbance increment of 0.01428 units/min at 260 nm at room temperature using 60 µg⁄mL of total yeast RNA as substrate.

We also now offer Ambion™ RNA Grade RNases A, V1 and T1 for RNA structure/function studies. For more information, see: AM2274 RNase A (1 µg⁄mL), AM2275 RNase V1 (0.1 U⁄µL), AM2283 RNase T1 (1 U⁄µL).